Effects of Copper / Zinc-Containing Superoxide Dismutase (Cu, Zn-SOD) and Catalase on Paraquat-Induced Injury in Primary Cultured Rat Skin Fibroblast

일차 배양한 백서 피부섬유아세포에서 Paraquat 독성에 미치는 SOD 와 Catalase 의 영향

  • 차종희 (조선대학교 의과대학 생화학교실) ;
  • 유의경 (세종대학교 화학과)
  • Published : 19940100


The participation of superoxide in initiating tissue damage derived from xenobiotics is best illustrated by paraquat intoxication. In the present study, the roles of superoxide dismutase and catalase on paraquat-induced cell injury were investigated using primary cultured rat skin fibroblast. The degree of cell injury was assassed by the conversion of reduced MTT to a blue formazan. Paraquat produced concentration-and time-related cell injury in cultured rat skin fibroblast. Paraquat induced-cell injury was aggravated by pretreatment of aminotriazol (AT: 10 mM), an catalase inhibitor, and attenuated by addition of catalase (100∼500 unit/ml). However, the effects of diethyldithiocarbamate (DDC : 10 mM), copper- and zinc-containing superoxide dismutase (Cu, Zn-SOD) inhibitor, and Cu, Zn-SOD on paraquat-induced injury were not significant. These results suggest that hydrogen peroxide might be more responsible factor than superoxide in the pathogenesis of paraquat-induced cell injury.



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