Isolation and Characterization of a Mutant Defective in Light-activated Heterotrophic Groth from Synechocystis sp. PCC 6803

Synechocystis sp. PCC 6803으로부터 광활성 종속영양 생장결핍 돌연변이체의 분리 및 특성

  • Park, Mi-Seon (Department of Microbiology, Chungbuk National University) ;
  • Lee, Young-Sook (Department of Microbiology, Chungbuk National University) ;
  • Kim, Young-Chang (Center for Molecular Microbiology, Seoul National University)
  • 박미선 (충북대학교 자연과학대학 미생물학과) ;
  • 이영숙 (충북대학교 자연과학대학 미생물학과) ;
  • 김영창 (서울대학교 분자미생물학과 연구센타)
  • Published : 1994.01.01

Abstract

A mutant strain PRM1 defective in light-activated heterotrophic growth was isolated from Synechocystis sp. PCC 6803. PRM1 could be grown at growth rate equivalent to Synechocystis 6803 under mixotrophic growth conditions. However, PRM1 could not be grown under light-activated heterotrophic conditions, in which a daily pulse of light for 5 min was given. These results suggest that PRM1 is not defective in heterotrophic metabolism, but in the transduction pathway of light signal essential to the growth. Plasmid patterns, absorption spectra of whole cells, and the exterior and interior structures of PRM1 were similar to those of Synechocystis 6803, except that PRM1 could not produce amorphous slime holding cells together.