Tissue-Based Amperometric Biosensor for Determination of L-Ascorbic Acid in Organic Media

유기 용매 속에서 L-Ascorbic Acid의 정량을 위한 바이오센서

  • Kwon, Hyo-Shik (Department of Science Education, College of Education, Chungbuk National University) ;
  • Lee, Cheal-Gyu (Department of Environmental Engineering, Chongju University)
  • 권효식 (충북대학교 사범대학 과학교육과) ;
  • 이철규 (청주대학교 이공대학 환경공학과)
  • Received : 1993.11.08
  • Published : 1994.06.25


An amperometric sensor for L-ascorbic acid(AA) in methanol media has been made by immobilizing squash-tissues on a graphite rod disk. A detection limit of the electrode was $2{\times}10^{-6}M$ L-ascorbic acid. In comparison with an isolated enzyme based ascorbate oxidase(AO) electrode, the plant-tissue electrode offered high biocatalytic stability and activity and extremely low cost. The electrode has a useful lifetime of 1 week.


Supported by : 충북대학교