Tissue-Based Amperometric Biosensor for Determination of L-Ascorbic Acid in Organic Media

유기 용매 속에서 L-Ascorbic Acid의 정량을 위한 바이오센서

  • Received : 1993.11.08
  • Published : 1994.06.25

Abstract

An amperometric sensor for L-ascorbic acid(AA) in methanol media has been made by immobilizing squash-tissues on a graphite rod disk. A detection limit of the electrode was $2{\times}10^{-6}M$ L-ascorbic acid. In comparison with an isolated enzyme based ascorbate oxidase(AO) electrode, the plant-tissue electrode offered high biocatalytic stability and activity and extremely low cost. The electrode has a useful lifetime of 1 week.

Keywords

Amperometric Biosensor;L-Ascorbic Acid(AA);Ascorbate oxidase(AO);Squash;Graphite rod disk

Acknowledgement

Supported by : 충북대학교