A Study on the Measurement of Dissociation Constant of Organic Acid with FIA

FIA에 의한 유기산의 해리상수 측정에 관한 연구

  • Received : 1994.03.17
  • Published : 1994.06.25

Abstract

The point of present study was focused on developing the method for the determination of dissociation constant by means of FIA conjunction with Diode Array Spectrphotometry or spectrophotometry along with small amount of Bromocresol Green(mg order) within short time. On the calculation with computer, the indicator with pK=7 has been shown the most sensitive reaction when little amount of base or acid has been added. The pKa of Bromothymol Blue and Bromocresol Green were measured as 7.31 and 4.82 respectively with spectrophotometry after activity correction by Kielland method (Reported value by Bishop, 7.30 and 4.79). The pKa of Bromocresol Green by FIA was obtained as 4.78 and was comparable with other values determined by other methods.

Keywords

FIA;dissociation conestant;Bromothymol Blue;Bromcresol Green