Determination of Sodium Chondroitin Sulfate by Enzymatic Digestion and HPLC

효소분해와 HPLC를 이용한 황산콘드로이틴나트륨의 정량

  • Kang, Seong-Ho (Research & Development Center of Hanil Pharmaceutical Ind. Co., LTD.) ;
  • Shin, Hoon (Research & Development Center of Hanil Pharmaceutical Ind. Co., LTD.) ;
  • Chang, Sun-Ki (Research & Development Center of Hanil Pharmaceutical Ind. Co., LTD.) ;
  • Yoon, Hyung-Jung (Research & Development Center of Hanil Pharmaceutical Ind. Co., LTD.)
  • 강성호 (한일약품(주) 중앙연구소) ;
  • 신훈 (한일약품(주) 중앙연구소) ;
  • 장순기 (한일약품(주) 중앙연구소) ;
  • 윤형중 (한일약품(주) 중앙연구소)
  • Received : 1994.04.15
  • Published : 1994.06.25

Abstract

In order to determine sodium chondroitin sulfate in the mixture, chondroitinase ABC was used for enzymatic reaction. The procedure was rapid, simple, quantitative and the HPLC analysis of ${\Delta}Di-6S$(2-actamido-2-deoxy-3-0-(${\beta}$-D-gluco-4-enepyranosyluronic acid)-6-0-sulfo-D-galactose) in the sodium chondroitin sulfate was obtained. The absorbance was measured at 230nm and detection limit was $1{\mu}g/ml$. When we applied this method to the drugs(capsule, opthalmic solution), it gave the mean contents of $100.01{\pm}1.58%$ and $99.89{\pm}1.80%$ respectively.

Keywords

Sodium chondroitin sulfate;chondroitinase ABC;HPLC;enzymatic reaction