# 신속한 BOD 측정장치의 개발과 응용

• 손무정 (계명대학교 자연과학대학 화학과) ;
• 김은정 (계명대학교 자연과학대학 화학과) ;
• 장세용 (계명대학교 자연과학대학 화학과) ;
• 홍대일 (계명대학교 자연과학대학 화학과)
A microbial BOD sensor for the continuous estimation of BOD was been developed by immobilizing Trichosporon cutaneum, which was immobilized between a dialysis and a gas-permeable membrane, on an oxygen electrode. The optimum pH and temperature for BOD measurement using this sensor were pH 7.0 and $32{\sim}33^{\circ}C$, respectively. The best result was obtained at 2~3ml/min flow rate in 0.1M phosphate buffer solution. A linear relationship was observed between ${\Delta}DO$ and the concentration of standard GGA solution below 60mg/l(90ppm $BOD_5$). The reproducibility was found to be within 3% for the standard solution containing glucose 30mg/l and glutamic acid 30mg/l. The output DO value of this sensor was almost constant for 30 dalys. The response time and the recovery time were about 5 and 10 min, respectively. This sensor was employed for the BOD measurement of waste pollutants and was compared with $BOD_5$ method.