The Elution Behaviors of Some Metal-2-Hydroxy-arylazopyrazolone Chelates by Reversed Phase Liquid Chromatography (II)

역상 액체 크로마토그래피에 의한 금속-2-hydroxy-arylazopyrazolone 유도체 킬레이트의 용리거동에 관한 연구 (II)

  • Lee, Won (Department of Chemistry, Kyunghee University) ;
  • Kim, In-Whan (Department of Chemistry, Education, Taegu University) ;
  • Kang, Chang-Hee (Department of Chemistry, Cheju National University) ;
  • Kim, Eun-Kyung (Department of Chemistry, Kyunghee University)
  • 이원 (경희대학교 문리과대학 화학과) ;
  • 김인환 (대구대학교 사범대학 화학교육과) ;
  • 강창희 (제주대학교 자연과학대학 화학과) ;
  • 김은경 (경희대학교 문리과대학 화학과)
  • Received : 1994.08.29
  • Published : 1994.09.25

Abstract

The elution behaviors in reversed-phase liquid chromatography were investigated thermodynamically for 2-hydroxy-arylazopyrazolone chelates with Ni(II), Cu(II), Co(III), Cr(III) on Novapak $C_{18}$ column. There was a good linear dependence of the capacity factor(k') on the variations of column temperature in van't Hoff plot. From this result, it was confirmed that the retention mechanism of these chelates in the reversed phase liquid chromatography system was invariant under the condition of various temperatures. For the most cases of the chelates studied, the dependence of capacity factor(1n k') on enthalpy$(-{\Delta}H)^{\circ}$, calculated by van't Hoff plot showed a good linearity(r=0.980~0.999) except [Pm(2-OH_(5-Cl)PaPz](r=0.787) and also the compensation temperatures(${\beta}$) showed constant values. The range of compensation temperature values calculated from the slope of $-{\Delta}H^{\circ}$ vs 1n k' plots was 374.3~806.9K. It was suggested that the retention of metal-2-hydroxy-arylazopyrazolone chelates in the reversed phase liquid chromatography system was largely affected by the hydrophobic effect.

Keywords

Metal-2-hydroxy-arylazopyrazolone Chelates;Reversed phase liquid chromatography;Elution behaviors;Retention mechanism

Acknowledgement

Supported by : 한국과학재단