SEPARATION, IDENTIFICATION OF BIOACTIVE COMPOUNDS FROM ALFALFA PLANT

알팔파의 생리활성물질 분리 및 동정

  • Received : 1994.08.29
  • Published : 1994.09.25

Abstract

To isolate, purity and identify of bioactive compounds involved in alfalfa allelopathy and/or autotoxicity, experiment was conducted. Isolation and separation procedures used from an 80% methanol extract of fresh alfalfa leaves(1kg), silica gel thin layer chromatography(TLC), followed by Droplet Counter Current Chromatography(DCCC). Preliminary identification was examined by high preformance lipid chromatography(HPLC). Four phenolic compound, salicylic acid, scopoletin, rutin, and quercetin, were identified and identified all compounds were phytotoxic to alfalfa seed germination and seedling growth. Among these compounds, quercetin treatment($10^{-3}M$) was most inhibitory to alfalfa seed germination and seedling growth. These compounds may be, at least in part, involved autotoxicity and allelopathy.

Keywords

Bioactive compounds;Autotoxicity;Allelopathy;Droplet counter current chromatography(DCCC)