Microanalysis of Vecuronium Bromide in Biological Fluids by Spectrofluorimetry and HPLC-Fluorescence Detection

형광광도법 및 HPLC-형광검출법에 의한 생체시료 중의 Vecuronium bromide의 미량분석

  • Received : 1994.07.18
  • Published : 1994.09.25

Abstract

The determination of the neuromuscular blocking agents vecuronium bromide(VeBr) in biological fluids has been investigated. The method depends on the formation of insoluble red complex between vecuronium bromide and rose bengal in aqueous layer. The amount of vecuronium bromide was calculated from that of extracted rose bengal which was determined by spectrofluorimetry or HPLC/fluorescence detection method. It was possible to analyze VeBr in the range of $2{\sim}32{\mu}g/ml$(r=0.998 for water soln., 0.999 for urine, 0.996 for plasma). This method was applied to the analysis of VeBr in biological fluids, urine and plasma.

Keywords

Vecuronium bromide;Rose bengal;Spectrofluorimetry;HPLC analysis