Analysis of Carbendazim and Carbaryl by Micro-HPLC

Micro-HPLC에 의한 Carbendazim과 Carbaryl의 분석

  • Choi, Kyoung-Sook (Young-In Research Institute of Science and Technology) ;
  • Lee, Hoe-Yeon (Young-In Research Institute of Science and Technology) ;
  • Lee, Duck-Hee (Young-In Research Institute of Science and Technology) ;
  • Byun, Woon-Ki (Young-In Research Institute of Science and Technology)
  • Received : 1994.06.23
  • Published : 1994.09.25

Abstract

A micro-high performance liquid chromatography(Micro-HPLC) techniquie with solid phase extraction was reported which detected carbendazim and carbaryl at picogram levels. They were separated on microbore packed $C_{18}$ column($1.0mm\;I.\;D{\times}150mm$, $d_f=5{\mu}m$) using a 50% methanol mobile phase and detected at UV 220nm(${\alpha}=2.94$, $R_s=4.71$), while they were not resolved on analtical HPLC system(${\alpha}=1.27$, $R_s=0.76$). The detection thresholds of carbendazim and carbaryl were 0.5ng and 0.1ng on Micro-HPLC, therefore Micro-HPLC system was 20~40 told more sensitive than anayltical HPLC system. Sep-Pak $C_{18}$ catridge was found to be efficient in enriching carbendazim and carbaryl from dilute aqueous solution with 97.0% and 97.8% recoveries of them. The Sep-Pak $C_{18}$ catridge followed by the Micro-HPLC had been applied to the quantitative analysis of carbendazim and carbaryl in spiked juices and a commercial drinking water.

Keywords

Micro-HPLC;microbore packed $C_{18}$ column;carbendazim;carbaryl;Sep-Pak $C_{18}$ catridge