The effect of thiamin on fetal growth and development in CD-1 mice exposed with mercury for the gestation period

임신 중 수은을 섭취한 CD-1 마우스 태아의 성장발육과 기형발생에 미친 티아민의 효능 평가

  • Kim, Jin-suk (Department of Veterinary Medicine and Animal Resources Research Centre, Kon-Kuk University) ;
  • Choi, Seok-wha (College of Veterinary Medicine, Chungbuk University)
  • 김진석 (건국대학교 수의학과 및 동물자원연구센타) ;
  • 최석화 (충북대학교 수의과대학)
  • Received : 1994.01.25
  • Published : 1994.01.31

Abstract

Pregnant CD-1 mice were exposed to methylmercury in the drinking water at concentration of 20ppm with subcutaneous treatment of thiaminHCl(vitamin $B_1$) (100mg, 200mg or 300mg/ kg b.w.) or BAL(5.0 mg/kg b.w.) under the alone or combined base at the therapeutic agents from day 6 to 15 of gestation. Fetal growth parameters, including body weight and crown-rump length in the mice exposed to mercury, were reduced as placental weight compared to those in the control group(no treatment). The incidence of dead fetuses/resorption and malformed fetuses(especially cleft palate) was also increased even in the group treated with thrapeutic agents as well as in the mercury only treated group. However, all kinds of alteration indicated above, possibly induced by mercury, reduced/or decreased significantly compared to those of control. A subtle indication of maternal toxicity was noted in most experimental animals as evidenced by decreased water consumption and increased relative liver weight. The present study confirmed that methylmercuric chloride is embrytoxic and teratogenic in CD-1 mice when administered during organogenesis and that thiamin administration may have therapeutic application for the treatment or prevention against of deleterious effects induced by mercury during gestation period.

Acknowledgement

Supported by : Animal Resources Research Center