Activity of myeloperoxidase and leukocyte peroxidase according to ages in the hen(Dekalbwarren)

산란계(Dakalbwarren)의 연령에 따른 myeloperoxidase와 leukocyte peroxidase 활성에 관한 연구

  • Chon, Seung-ki (College of Veterinary Medicine, Chonbuk National University) ;
  • Kang, Chang-won (College of Veterinary Medicine, Chonbuk National University) ;
  • Lee, Ho-il (College of Veterinary Medicine, Chonbuk National University)
  • 전승기 (전북대학교 수의과대학) ;
  • 강창원 (전북대학교 수의과대학) ;
  • 이호일 (전북대학교 수의과대학)
  • Received : 1994.07.19
  • Published : 1994.07.30

Abstract

This study was undertaken to measure the activity of myeloperoxidase and leukocyte peroxidase of hen. 70 hens were decapitated to observe the activity of enzymes according to ages. The activity of peroxidase by the Lowry's method with bovine serum albumin as standard. Gel filtration chromatography was carried out of sephacryl S-300 column. The results obtained were summarized as follows; 1. The mean of specific activity of myeloperoxidase and leukocyte peroxidase was 16.80(units/mg) and 15(units/mg), respectively. 2. The specific activity of myeloperoxidase in 35 days hen was significantly increased and showed almost the same level of activity to 350 days hen. 3. The specific activity of leukocyte peroxidase in 35 days hen was significantly increased and showed a little increased tendency from 210 days to 350 days hen. 4. On the sephacryl S-300 column chromatography, two separated peaks of myeloperoxidase activity were observed. The molecular weights of myeloperoxidase were 57,000 dalton and 13,700 dalton.