Actions of acetylcholine, norepinephrine, histamine and prostaglandin F2α on motility of pig oviductal isthmic smooth muscle

돼지 난관협부 평활근의 운동성에 대한 acetylcholine, norepinephrine, histamine 및 prostaglandin F2α의 작용

  • Rho, Gyu-jin (College of Veterinary Medicine, Gyeongsang National University) ;
  • Park, Sang-eun (Central Branch, Gyeongnam Veterinary Medicine) ;
  • Shim, Cheol-soo (Eastern Branch, Gyeongnam Veterinary Medicine) ;
  • Kim, Joo-heon (College of Veterinary Medicine, Gyeongsang National University) ;
  • Choe, Sang-young (College of Veterinary Medicine, Gyeongsang National University)
  • 노규진 (경상대학교 수의과대학) ;
  • 박상은 (경남 가축위생시험소 중부지소) ;
  • 심철수 (경남 가축위생시험소 동부지소) ;
  • 김주헌 (경상대학교 수의과대학) ;
  • 최상용 (경상대학교 수의과대학)
  • Received : 1994.07.30
  • Published : 1994.07.30

Abstract

The purpose of this study was to investigate the effects of neurotransmitters and the source of $Ca^{2+}$ in the effects of neurotransmitters on the motility of pig oviductal isthmic smooth muscle. The motility of the isolated smooth muscle was recorded by using physiological recording system. The results were summarized as follows; Acetylcholine, norepinephrine, histamine and prostaglandin $F_{2{\alpha}}(PGF_{2{\alpha}})$ caused the contraction and the contractile responses were increased in a dose-dependent manner from the concentration of $10^{-7}$ to $10^{-4}M$. The maximum contractility of acetylcholine, norepinephrine, histamine and $PGF_{2{\alpha}}$ was 65.99, 28.66, 83.99 and 47.33% of 100 mM K contraction, respectively. The contractile response induced by acetylcholine$(10^{-6}M)$ was completely blocked by the pretreatment with cholinergic receptor blocker, atropine$(10^{-6}M)$, the contractile response induced by norepinephrine$(10^{-5}M)$ was blocked by the pretreatment with ${\alpha}$-adrenergic receptor blocker, phentolamine$(10^{-6}M)$ but was not blocked and rather increased by the pretreatment with ${\beta}$-adrenergic receptor blocker. propranolol$(10^{-6}M)$, the contractile response induced by histamine$(10^{-6}M)$ was completely blocked by the pretreatment with $H_1$-histaminergic receptor blocker, pyrilamine$(10^{-6}M)$ but was increased by the pretreatment with $H_2$-histaminergic receptor blocker, cimetidine$(10^{-6}M)$. The contractile response induced by acetylcholine$(10^{-6}M)$, norepinephrine$(10^{-5}M)$ and histamine$(10^{-6}M)$ was weakly contracted response in $Ca^{2+}$-free medium, but the contractile response induced by $PGF_{2{\alpha}}(10^{-6}M)$ was disappeared. The contractile response induced by acetylcholine$(10^{-6}M)$, norepinephrine$(10^{-5}M)$ and histamine$(10^{-6}M)$ was powerfully depressed by the pretreatment with $Ca^{2+}$-channel blocker, verapamil$(10^{-5}M)$ but the contractile response induced by $PGF_{2{\alpha}}(10^{-6}M)$ was completely inhibited.