The carcinogenicity study of Folpet in rats

랫드에서 Folpet의 발암성에 관한 연구

  • Lee, Yong-soon (College of Veterinary Medicine, Seoul National University) ;
  • Cho, Jae-jin (College of Veterinary Medicine, Seoul National University) ;
  • Kang, Kyung-sun (College of Veterinary Medicine, Seoul National University) ;
  • Kim, Bae-hwan (College of Veterinary Medicine, Seoul National University) ;
  • Nam, Ki-hoan (College of Veterinary Medicine, Seoul National University) ;
  • Seo, Kwang-won (College of Veterinary Medicine, Seoul National University) ;
  • Kang, Seong-keun (College of Veterinary Medicine, Seoul National University) ;
  • Lim, Yun-kyu (Department of Veterinary Medicine, Cheju National University) ;
  • Heo, Kang-jun (College of Veterinary Medicine, Chungbuk National University)
  • Received : 1994.05.13
  • Published : 1994.07.30

Abstract

This study was performed for assessing carcinogenicity of Folpet using medium-term carcinogenicity bioassay. Sprague-Dawley rats aged six weeks divided into four grout's and were initially given an intraperitoneal injection of diethylnirosamine at 200mg/kg body weight. Two weeks later, group 1(negative control) was treated with basal diet. A Folpet was given per oral administration to group 2(100 ppm) and goup 3(1,000 ppm). Group 4 was fed on water containing 0.05% phenobarbital sodium as a promtor for six weeks. At three weeks after beginning of the experiment, partial hepatectomy was performed in all rats. The tumor-promoting effects were examined by the numbers and areas per $cm^2$ of induced glutathion S-tranferase placetal form(GST-P) positive foci in liver, and silver stained nucleolar organizer regions(AgNORs) which have recently introduced as one of the indicators for the cell proliferative activity. As the results, Folpet didn't have tumor-promoting effects on GST-P positive foci developement and AgNORs during promoting stage after initiation, whereas phenobarbital sodium treatment group showed promoting effect. It was concluded that Folpet didn't have promoting effect at 500, 1,000 ppm using this midium-term carcinogenicity bioassay model.