Characteristics of Nitrite Scavenger Derived from Seeds of Cassia tora L.

결명자 추출물의 아질산염 소거인자의 특성

  • Park, Yeung-Beom (Department of Food Science and Technology, National Fisheries University of Pusan) ;
  • Lee, Tae-Gee (Department of Food Science and Technology, National Fisheries University of Pusan) ;
  • Kim, Oi-Kyung (Department of Food Science and Technology, National Fisheries University of Pusan) ;
  • Do, Jeong-Ryong (Korea Food Research Institute) ;
  • Yeo, Saeng-Gyu (Department of Food Processing, Pusan Junior College) ;
  • Park, Yeung-Ho (Department of Food Science and Technology, National Fisheries University of Pusan) ;
  • Kim, Seon-Bong (Department of Food Science and Technology, National Fisheries University of Pusan)
  • 박영범 (부산수산대학교 식품공학과) ;
  • 이태기 (부산수산대학교 식품공학과) ;
  • 김외경 (부산수산대학교 식품공학과) ;
  • 도정룡 (한국식품개발연구원) ;
  • 여생규 (부산전문대학 식품가공과) ;
  • 박영호 (부산수산대학교 식품공학과) ;
  • 김선봉 (부산수산대학교 식품공학과)
  • Published : 1995.02.01

Abstract

The present study was conducted to elucidate nitrite scavenger derived from seeds of Cassia tora L. Methanolic extract of Cassia tora L was refractionated into ethyl ether, chloroform, ethyl acetate and water farction, and nitrite scavenging abilities of these fractions were investigated. Among these fractions, ethyl acetate fraction had the strongest nitrite scavenging ability(97%/2 mg). Therefore, to further investigate nitrite scavenger, ethyl acetate fraction was fractionated by silica gel column chromatography with a chloroform-methanol($10:0{\sim}0:10$) and then compound A and B were isolated. Compound A had a stronger nitrite-scavenging effect than compound B. Therefore the further separation of compound A was carried out by HPLC(32% acetonitrile. 1 ml/min) using ${\mu}Bondapak$C18 column$(3.9{\times}300 mm)$ and finally compound A-1 was obtained from compound A. Compound A-1 had by far nitrite-scavenging ability as compared with that of ascorbic acid. Compound A-1 was identified as $nor-rubrofusarin-6-{\beta}-mono-D-glucoside$ from the profiles of UV, IR and $^1H-NMR$.

Keywords

nitrite scavenger;Cassia tora L..;$nor-rubrofusarin-6-{\beta}-mono-D-glucoside$