Studies on isolation of rhinopneumonitis virus from Korean horses and its immunogenicity III. Studies on immunogenicity of equine herpesvirus in experimental animals

국내 말로부터 비폐렴바이러스의 분리 및 면역원성에 관한 연구 III. 말 비폐렴바이러스의 실험동물에 대한 면역원성 조사

  • Cho, Gil-jae (Korean Racing Association, Cheju Race Course) ;
  • Kim, Bong-hwan (College of Veterinary Medicine, Kyungpook National University) ;
  • So, Sin-hee (College of Veterinary Medicine, Kyungpook National University) ;
  • Lee, Du-sik (Department of Veterinary Medicine, Cheju National University)
  • Received : 1995.05.09
  • Published : 1995.10.30

Abstract

The study was carried out to investigate the pathogenicity of EHV isolate to hamsters and mice and immunogenicity of experimentally produced. vaccine were evaluated in the horses. Hamsters infected. intranasally with $LC_1$ isolate showed symptoms of nasal discharge, conjunctivitis and body weight loss during the observation period of 12 days after infection, while only slight depression and body weight loss were noticed with mice infected with $LC_1$ indicating that hamsters are more susceptible to the virus. Antibody titer of mice and hamsters were gradually increased to highest level of 1:2560~10240, 1:640~1280, respectively, at 7~12 days post vaccination. Horses immunized against $LC_1$ killed vaccine reached to maximum antibody titer of 1:20480 around 4 weeks after 1st vaccination and declined after 12 weeks post vaccination. No significant antibody increase were detected after 2nd vaccination. Mean body temperature and mean total leukocyte counts remained within normal range and no adverse reaction were noticed after vaccination.

Acknowledgement

Supported by : 한국학술진흥재단