Production of monoclonal antibody(MAb) for lymphocytes of Korean native cattle 1. MAb to T lymphocytes

한우 림프구에 대한 단일항체 생산 1. T 림프구에 대한 단일항체

  • Song, Hee-jong (College of Veterinary Medicine, Chonbuk National Universtiy) ;
  • Woon, Jae-ho (College of Veterinary Medicine, Chonbuk National Universtiy) ;
  • Kim, Tae-joong (College of Veterinary Medicine, Chonbuk National Universtiy) ;
  • Chai, Hyo-seok (Chonbuk Veterinary Service Laboratory) ;
  • Hur, Boo-hong (Chonbuk Veterinary Service Laboratory) ;
  • Choi, Min-soon (College of Ocean Science and Technology, Kunsan National University)
  • 송희종 (전북대학교 수의과대학) ;
  • 운재호 (전북대학교 수의과대학) ;
  • 김태중 (전북대학교 수의과대학) ;
  • 채효석 (전라북도 가축위생시험소) ;
  • 허부홍 (전라북도 가축위생시험소) ;
  • 최민순 (군산대학교 해양산업대학)
  • Received : 1995.07.14
  • Published : 1995.10.30

Abstract

This study was undertaken to develop the monoclonal antibody(MAb) for lymphocytes of Korean native cattle by the cell hybridization of myeloma P3/NS-1/ 1-Ag-4-1 and spleen cells from BALB/c mice hyperimmunized with nylon wool column eluted peripheral T lymphocytes of Korean native cattle. The isotype of MAb KCT-14 against T lymphocyte was mouse $IgG_1$. KCT-14 positivity of mononuclear cells(MNC) from peripheral blood lymphocytes, nylon wool nonadherent and adherent-lymphocytes was 41.7%, 58.4% and 22.6%, respectively. And that of mesenteric lymph node-, spleen and thymus-MNC was 43.3%, 40.2% and 33.6%, respectively. Immunoperoxidase staining of frozen tissue sections showed that the MAb positive cells were located in the medulla of the thymus and in the paracortical area and the mantle zone of the germinal center in the lymph nodes. These results indicated that KCT-14 was one of the MAb for investigate of T lymphocyte subpopulations in the Korean native cattle.

Acknowledgement

Supported by : 한국과학재단