- Volume 20 Issue 2
Comparison of 4 Methods of DNA Extraction for Sex Determination and D1S80 Locus Detection in Teeth
치아를 이용한 성별검사 및 D1S80 유전좌위의 검색시 4가지 DNA추출방법에 따른 비교
- Woong Hur (Dept. of oral Medicine and Diagnosis, College of Dentistry, Chonnam National University) ;
- Chang-Lyuk Yoon (Dept. of oral Medicine and Diagnosis, College of Dentistry, Chonnam National University)
- Published : 1995.06.01
Human genomic Deoxyribonucleic acid(DNA) was extracted from teeth by boiling, salting-out, phenol, boiling-phenol methods. The author compared DNA concentration and its purity, the accuracy of sex determination and the results of the D1S80 locus detection among above 4 methods. The following results were obtained : 1. DNA concentration was the highest in pulp with salting-out method and DNA purity was higher in pulp with salting-out and phenol methods than other 2 methods. 2. Sex determination was possible using of the pulp and the dentin of the teeth with four methods but, it was impossible in the enamel and some pulp with boiling method. 3. Amplification of D1S80 locus occurred from pulp and dentin with salting-out, phenol, and boiling-phenol methods. 4. There are no differences among the amplification of X-Y homologus amelogenin gene by application of 4 methods and salting-out, phenol methods efficiently makes available to amplification of D1S80 locus. From the investigation DNA extraction, sex determination, amplification of D1S80 locus was successfully accomplished with salting-out, phenol, boiling-phenol methods Therefore above 3 methods are available and applicable as forensic odontology for individual identification.