Analysis of the DNA Fingerprints from the Teeth -Using Chelex$^\textregistered$ 100 as a Medium of Simple Extraction of DNA from the Teeth-

치아에서의 DNA 유전자지문 분석 -Chelex$^\textregistered$ 100을 매개체로 한 DNA추출-

  • Chang-Lyuk Yoon (Dept. of Oral Diagnosis and Oral Medicine, College of Dentistry, Chosun University)
  • Published : 1995.06.01

Abstract

The human genomic deoxyribonucleic acid(DNA) was extracted from the pulp, dentin of 22 teeth by clelex, phenol methods. Samples of the tooth-derived DNA were amplified by polymerase chain reaction(PCR), electrophosed for sex determination by detection of X-Y homologus amelogenin gene and D1S80 locus detection The following results have been achieved. 1. Chelex and phenol method are effective to sex determination in the pulp and dentin 2. Chelex method is not suitable for detection of D1S80 locus. 3. Concentration and purity of DNA for teeth using chelex method is lower than using phenol method. From the above investigation, chelex method is simple, rapid for sex determination, but it is not suitable for detection of VNTRs.