Reaction Mechanixm of Cyclodextrin formation from Swollen Extrusion Starch by cyclocextrin Glucanotransferase

팽윤 전분을 기질로 한 Cyclodextrin Glucanotransferase의 Cyclodextrin 생성반응 기작

  • 이용현 (경북대학교 자연과학대학 유전공학과) ;
  • 조명진 (경북대학교 자연과학대학 유전공학과) ;
  • 박동찬 (경북대학교 자연과학대학 유전공학과)
  • Published : 1995.08.01


Mechanism of the cyclodextrin (CD) production reaction by cyclodextrin glucanotransferase (CGTase) using swollen extrusion starch as substrate was investigated emphasizing the structural features of starch granule. The degree of gelatinization was identified to be the most representative structural characteristic of swollen starch. The most suitable degree of gelatinization of swollen starch for CD production was around 63.52%. The structural transformation of starch granule during enzyme reaction was also followed by measuring the changes of the degree of gelatinization, microcrystallinity, and accessible and inaccessible portion to CGTase action of residual swollen starch. The adsorption phenomenon of CGTase to swollen starch was also examined under various conditions. The inhibition mechanism of CGTase by various CDs was identified to be competitive, most severely by a-CD. The mechanism elucidated will be used for development of a kinetic model describes CD production reaction in heterogeneous enzyme reaction system utilizing swollen extrusion starch.



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