- Volume 24 Issue 3
Effects of Salt and Heparin on the Activation of Lipoprotein Lipase(LPL)
- Kim, Tae-Woong (Dept. of Biochemistry, Kangwon National University) ;
- Lee, Jae-Bok (Dept. of Biochemistry, Kangwon National University) ;
- Son, Heung-Soo (Institute of Biotechnology, Korea University) ;
- Kim, Sung-Wan (Dept. of Biochemistry, Kangwon National University)
- Published : 1995.06.01
Lipoprotein lipase(LPL) is an acylglycerol hydrolase and is the extrahepatic enzyme responsible for the hydrolysis of triglyceride-rich plasma lipoproteins. LPL has been isolated from bovine milk by affinity chromatography on heparin-sepharose in 2M NaCl, 5mM barbital buffer, pH 7.4. Para-nitrophenyl butyrate(PNPB) was used as a substrate for the determination of LPL activity. Molecular weight of LPL was 55KD on 10% SDS-PAGE. When the effects of heparin on LPL activation were compared, LPL activity of heparin added group increased approximately 5 times higher than that of heparin non-added groups. These results indicated that heparin involved in the stabilization of LPL structure that led to increase enzyme activity. Furthermore, LPL activity increased about 4 times compared to the absence of heparin at various pH. LPL was stabilized when heparin was added either low or high salt concentrations. With the presence of heparin, NaCl concentration did not affect LPL activity at pH range 6∼9.
- J. Lipid. Res. v.32 Distribution and source of lipoprotein in mouse mammary gland. Jensen,D.R.;Bessessen,D.H.;Etienne,J.;Eckel,R.H.;Neville,M.C.
- J. Lipid Res. v.33 Structure and evolution of the lipase superfamily. Hide,W.A.;Chan,L.;Li,W.H.
- Biochim. Biophys. Acta. v.1169 Lipoprotein lipase gene expression :Physiological regulators at the transcriptional and post-transcriptional level. Enerback,S.;Gimble,J.M.
- Ann. Rev. Biochem. v.48 Lipolytic enzymes and plasma lipoprotein metabolism. Nilsson-Ehle,D.;Garfimkel,A.S.;Schotz,M.C.
- Biochem. J. v.262 Lipid metabolism during the initiation of lactation in the rat. The effects of starvation and tumer growth. Evans,R.D.;Williamson,D.H.
- Adipose tissue lipoprotein lipase Lipoprotein lipase Eckel,R.H.;Borensztajn,J.(ed.)
- Methods for studying lipoprotein lipase in human adipose tissue v.II Methods in Diabetes Reserch Eckel,R.H.;Kern,P.A;Sadur,C.N.;Yost,T.J.;Clinical Methods Clarke,W.L.;Larner,J.;Pohl,S.L.(ed.)
- J. Biol. Chem. v.257 Lipoprotein lipase-catalyzed hydrolysis of PNPB. Shirai,K.;Jackson,R.L.
- J. Biol. Chem. v.257 Reciprocal effect of ApoC-Ⅱ on the LPL catalyzed hydroysis of PNPB and trioleoylglycerol. Shirai,K.;Jackson,R.L.
- J. Biol. Chem. v.265 Lipoprotein ApoC-Ⅱ activation of lipoprotein lipase ; Modulation by apolipoprotein A-Ⅳ Goldberg,I.J.;Scheradi,C.A.;Yacoub,L.K.;Saxena,U;Bisgaier,C.L
- N. Engl. J. Med. v.320 Lipoprotein lipase: a multifunctional enzyme relevant to common metabolic diseases. Eckel,R.H.
- Lipoprotein lipase Plasma Lipoproteins Garfinkel,A.S.;Schotz,M.C.;Gotto,Jr,A.M.(ed,)
- J. Biol. Chem. v.264 Structure of bovine milk lipoprotein lipase Yang,C.Y.;Gu,Z.W.;Yang,H.X.;Rhode,M.F.;Gotto,Jr.A.M.;Pownel,H.J.
- Biochemistry v.21 Lipoprotein lipase catalyzed hydrolysis of water soluble p-nitrophenyl esters. Inhibition by apolipoprotein C-II Quinn,D.M.;Shirai,K.;Jackson,R.L.;Harmong,J.A.K.
- Biochim. Biophys. Acta. v.1123 Structure and functional properties of lipoprotein lipase. Wang,C.S.;Hartsuck,J.;McConathy,W.J.
- J. Biol. Chem. v.251 Lipoprotein lipase from bovine milk lverius,P.H.;Ostlund-Lindgvist,A.M.
- J. Biol. Chem. v.262 Synthesis and secretion of lipoprotein lipase in 3T3-L1 adipocytes :demostration of inactive forms of lipase in cells Olivercroma,T.;Cheernick,S.S.;Bengtsson-Olivecrona,G.;Garrison,M.;Scow,R.O
- Ann. N.Y. Acad. Sci. v.556 Interaction of lipoprotein lipase with heparin Oka,K.;Wang-lverson,P.;Paterniti,Jr,J.R.;Brown,W.V.
- J. Biol. Chem. v.265 The response of lipoprotein lipase to feeding and fasting Doolittle,M.H.;Ben-Zeev,O.;Elovson,J.;Martin,D.;Kirchgessner,T.G.