Changes in Solution Properties of Pectins by Enzymatic Hydrolysis of Sidechains

  • Hwang, Jae-Kwan (Korea Food Research Institute, Kyonggi-do) ;
  • Jozef L. Kokini (Dept. of Food Science, Rutgers University)
  • Published : 1995.06.01

Abstract

The neutral sugar sidechains of apple pectins were hydrolyzed by commercial hemicellulase produced from Aspergillus niger, and the corresponding changes in solution viscosity were investigated in dilute(cc*) pectin solutions. Pectinase activity included in hemicellulases was removed by Epoxy-activated Sepharose 6B affinity chromatography using polygalacturonic acid as a ligand. Enzymatic hydrolysis of sidechains did not affect the specifc viscosity of dilute(0.5%) pectin solutions, while viscosity significantly decreased in concentrated(2.0∼6.0%) region. These results strongly suggest that the sidechains of pectins exists as an entangled state in concentrated solutions. It was also found that in the concentrated region the extent of viscosity reduction was dependent on pectin concentrations.

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