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MIGRATION OF THE PRIMORDIAL GERM CELLS AND GONAD FORMATION IN THE EARLY CHICKEN EMBRYO

  • Hong, Y.H. (Department of Animal Science and Technology, College of Agriculture and Life Sciences, Seoul National University) ;
  • Seo, D.S. (Department of Animal Science and Technology, College of Agriculture and Life Sciences, Seoul National University) ;
  • Jeong, D.K. (Department of Animal Science and Technology, College of Agriculture and Life Sciences, Seoul National University) ;
  • Choi, K.D. (Department of Animal Science and Technology, College of Agriculture and Life Sciences, Seoul National University) ;
  • Han, J.Y. (Department of Animal Science and Technology, College of Agriculture and Life Sciences, Seoul National University)
  • Received : 1995.01.26
  • Accepted : 1995.04.23
  • Published : 1995.12.01

Abstract

In this study, characteristics of chick primordial germ cells (PGCs), which is the founder cell of the germline, and gonadal development of the chick embryo between 12hrs and 6 day of incubation were investigated by transverse serial sections of chick embryos under the light microscopic observation. In embryo stage 20 (3 day of incubation), there are a lot of PGCs at the mesenchym, which were moving to the thickened epithelium (gonadal ridge). The PGCs arrive at both right and left gonad primordial in equal number prior to stage 24 (4 day of incubation), but in the following stages, the distribution of the PGCs became asymmetrical. More PGCs colonized the left than the right gonad, but the reason for the unequal distribution of PGCs is uncertain. The PGCs have mostly settled in the gonadal ridge (GR) at 6 day embryo. This study was conducted to investigate characteristics of the PGC migration and gonadal formation and observe the best condition for PGC isolation, culture and to attempt the possibility of the production for transgenic germline chimeras with manipulated PGCs.

Keywords

Primordial Germ Cell;Gonadal Ridge;Serial Section;Chick Embryo

Acknowledgement

Supported by : Korea Science and Engineering Foundation

Cited by

  1. Introduction of Foreign Gene into the Gonads via Primordial Germ Cells Originated from Germinal Crescent of Chicken Embryos vol.18, pp.1, 2000, https://doi.org/10.1080/09712119.2000.9706321