DOI QR코드

DOI QR Code

EFFECTS OF OVARY TYPE, OOCYTE GRADE, HORMONE, SPERM CONCENTRATION AND FERTILIZATION MEDIUM ON IN VITRO MATURATION, FERTILIZATION AND DEVELOPMENT OF BOVINE FOLLICULAR OOCYTES

  • Im, K.S. (Dept. of Animal Science and Technology, College of Agriculture and Life Sciences, Seoul National University) ;
  • Kim, H.J. (Dept. of Animal Science and Technology, College of Agriculture and Life Sciences, Seoul National University) ;
  • Chung, K.M. (Korea Biotechnology Institute) ;
  • Kim, H.S. (Livestock Experiment Station, Office of Rural Development) ;
  • Park, K.W. (Dept. of Animal Science and Technology, College of Agriculture and Life Sciences, Seoul National University)
  • Received : 1994.05.19
  • Accepted : 1994.11.03
  • Published : 1995.04.01

Abstract

In vitro embryo production (IVP) is affected by various factors during in vitro maturation, fertilization, and development. In this experiment, the effect of ovary type, quality of follicular oocyte, medium used for fertilization, presence of hormone in medium, sperm concentration on in vitro maturation and fertilization were examined for effective IVP. In vitro maturation was carried out using TCM-199 supplemented with 15% FCS and hormones in 5% $CO_2$ incubator for 24h. In vitro fertilization was performed with frozen-thawed sperm in modified mTALP medium containing 0.3% BSA, $10{\mu}g/ml$ heparin, and 5mM/ml caffeine for 24h. The fertilized embryos were co-cultured on monolayer of cumulus cells in TCM-199. When oocytes were collected from functionally active and inactive ovaries, maturation rate was 76.9 and 7.7%, respectively. When oocytes were classified morphologically to good and poor grades, maturation rate was 75 and 58.8%, respectively. FSH + LH + $E_2$ (86.4%) showed higher maturation rate than control (53.0%) and FSH (73%). The fertilization rate was 28.2, 100 and 91.7% in $1.6{\times}10^5$, $5.0{\times}10^5$ and $10.0{\times}10^5$ sperm concentration per ml. When oocytes were fertilized in mTALP and BO media, fertilization and cleavage rates of oocytes in mTALP were higher (84.3 and 56.9%) than those (67.4 and 23.3%) in BO medium. In this experiment, in vitro maturation, fertilization and development of oocytes were affected by type of ovary, grade of oocyte, hormones, sperm concentration and fertilization medium.

Keywords

Follicular Oocyte;In Vitro Matturation;In Vitro Fertilization;Hormone;Sperm Concentration

Acknowledgement

Supported by : Kon-Kuk University