The immunohistochemical study on the morphological changes of oxytocin secreting neurons in the age-related rat hypothalamus

고령에 따른 흰쥐 시상하부 oxytocin 분비세포의 형태적 변화에 대한 면역조직화학적 연구

  • Kim, Jin-sang (College of Rehabilitation Science, Taegu University) ;
  • Yi, Sung-joon (College of Veterinary Medicine, Kyungbuk National University)
  • 김진상 (대구대학교 재활과학대학) ;
  • 이성준 (경북대학교 수의과대학)
  • Received : 1994.10.27
  • Published : 1995.01.31

Abstract

This study was carried out to investigate the morphological changes of the oxytocin secreting neurons in age-related rat hypothalamus by means of immunohistochemistry. The experimental group was 20 aged female rats (Sprague-Dawley, 25~30 months), and the control group was 10 adult female rats (10 months). All animals were perfused transcardially with 4% paraformaldehyde-lysine-periocdate(PLP), and serial transverse brain sections($30{\mu}m$) were prepared by means of cryotome, and were stained with rabbit anti-oxytocin antisera immunohistochemically, using the free floating method and avidin-viotin peroxidase complex. The results were as followings. 1. The immunostained oxytocin secreting neurons were located at the paraventricular nucleus and supraoptic nucleus of age-related rat hypothalamus chiefly. 2. The numbers of oxytocin secreting neurons decreased at the paraventricular nucleus and supraoptic nucleus of age-related rat hypothalamus(p<0.01) 3. The oxytocin secreting neurons of age-related rat hypothalamus immunostained less than those of the adult rat hypothalamus, and the paraventricular nucleus immunostained greater than supraoptic nucleus in age-related rat. 4. The numbers and expansion of dendrite and axonal varicosities decreased in the age-related rat hypothalamus greatly.

Acknowledgement

Supported by : 학술진흥재단