Analysis of human HoxA gene control region and its effects on anterior-posterior axial pattern formation using transgenic mouse embryo

Transgenic mouse embryo를 이용한 human HoxA 유전자의 조절부위 분석과 전후축 형태형성(anterior-posterior axial pattern formation)에 미치는 영향

  • 장승익 (충남대학교 수의과대학) ;
  • 민원기 (한국과학기술연구원 유전공학연구소) ;
  • 박종훈 (한국과학기술연구원 유전공학연구소) ;
  • 이철상 (한국과학기술연구원 유전공학연구소) ;
  • 이경광 (한국과학기술연구원 유전공학연구소) ;
  • 이영원 (한국과학기술연구원 유전공학연구소) ;
  • 전무형 (충남대학교 수의과대학) ;
  • 김명희 (한국과학기술연구원 유전공학연구소)
  • Received : 1994.11.28
  • Published : 1995.01.31

Abstract

The human homolog of position specific element of mouse Hoxa-7 was studied using transgene. It contains a 1.1 kb human DNA (HCR)- a homolog to the intergenic region between Hoxa-7 and -9, which directs the position specific expression of Hoxa-7-, tk promoter, LacZ (${\beta}$-galactosidase) gene as a reporter, and polyadenylation signal of SV40 large T antigen. It was injected into the mice embryos, and the resulting transgenic embryos were analysed through PCR as well as genomic Southern blotting with placenta DNA. Out of 20 embryos analysed, two were transgenic. Among them, one transgenic embryo expressed transgene when stained with X-gal. The expression pattern was in analogy to that of the mouse Hoxa-7, showing spatially restricted expression pattern, Since the expression of ${\beta}$-galactosidase is regulated by the upstream human HCR sequence, it implies that the HCR is the plausible position specific regulatory element of human.