Serological cross-reaction with Brucella abortus antigen extracted by sodium dodecyl sulfate and Yersinia enterocolitica 0:9

SDS 처리한 브루셀라 항원과 Yersinia enterocolitica 0:9주의 혈청학적 교차반응 연구

  • Lim, Yoon-kyu (Department of Veterinary Medicine, Cheju National University) ;
  • Yang, Ki-chun (Department of Veterinary Medicine, Cheju National University) ;
  • Lee, Kyung-kap (Department of Veterinary Medicine, Cheju National University) ;
  • Park, Jun-hong (Department of Veterinary Medicine, Cheju National University) ;
  • Lee, Du-sik (Department of Veterinary Medicine, Cheju National University) ;
  • Park, Yong-ho (Veterinary Research Institute, RDA) ;
  • Kang, Seung-won (Veterinary Research Institute, RDA) ;
  • Mok, Ji-won (National Institute of Safety Research) ;
  • Lee, Yong-soon (College of Veterinary Medicine, Seoul National University)
  • Received : 1994.11.21
  • Published : 1995.01.31

Abstract

Brucella abortus cell wall antigen was extracted from Brucella abortus 1119-3 by ultrasonication and followed by sodium dodecyl sulfate(SDS) treatment. In order to confirm whether this preparation is serologically cross reactive with Yersinia enterocolitica 0:9, Western blot analysis with mouse anit-Brucella abortus1119-3 and with mouse anti-Yersinia enterocolitica 0:9 was performed. ELISA results from using those Brucella antigen and Yersinia antigen were assessed whether they had correlation. According to the results of western blot analysis and ELISA, there was no evidence of cross reactivity between the Brucella abortus 1119-3 antigen preparation and Yersinia enterocolitica 0:9. Therefore the SDS treated antigen prepared in this study could be suitably used as specific ELISA antigen without confusion in the interpretation of serological tests for brucellosis in cattle.

Acknowledgement

Supported by : 과학재단