Studies of the chromosome structure of tissue cultured different organisms from the rainbow trout

조직배양에 의한 무지개송어 장기조직세포의 염색체 기본구조에 관한 연구

  • Jung, Yung-rae (College of Veterinary Medicine, Chungnam National University) ;
  • Jeong, Young-gil (College of Veterinary Medicine, Chungnam National University) ;
  • Son, Jin-ki (Korea Ocean Reserch & Development Institute) ;
  • Kim, Moo-kang (College of Veterinary Medicine, Chungnam National University)
  • Received : 1995.02.03
  • Published : 1995.04.30

Abstract

This study was examined chromosome structure of kidney, fin, blood cell, sexual organ that were differently examined chromosome of a good cell activity from organ, medium, staining methods, instruments with rainbow trout 100. And so we obtained following conclusion. 1. The difference from each organ and medium is that a good cell activity of fin, kidney, sexual organ were obtained in TC-199 medium and a good cell activity in TC-199 medium+PHA(5%). 2. In the difference by staining methods, G-banding and C-banding were analyzed by electron microscope or cytoscan. Among them, heterochromatin of centromere analyzed by C-banding. 3. The zygotene and the leptotene were analyzed in this study. 4. Karyotype, heterochromatin and each stage of cell division were clearly analyzed by cytoscan.