Molecular cloning and nucleotide sequencing of bovine viral diarrhea virus gp53 antigenic region

소 바이러스성 설사병 바이러스 gp53 항원부위 유전자의 재조합 및 염기서열 연구

  • Yeo, Sang-geon (College of Veterinary Medicine, Gyeongsang National University) ;
  • Cho, HJ (Animal Diseases Research Institute, Agriculture Canada) ;
  • Masri, SA (Animal Diseases Research Institute, Agriculture Canada)
  • 여상건 (경상대학교 수의과대학) ;
  • ;
  • Received : 1995.01.05
  • Published : 1995.04.30

Abstract

Molecular cloning and nucleotide sequencing were undertaken for the RNA genome of gp53 antigenic region in cytopathic Singer strain of bovine viral diarrhea virus. The cloned cDNA was 939 nucleotides in length having a base composition of 31.0% A, 19.6% C, 25.5% G and 24.0% T. The sequence was corresponded to approximately 77.8%(817 bases) of predicted gp53 region and 122 bases after 3'end of gp53 region in the Singer strain when compared with NADL strain of known sequence. A single open reading frame was found in the sequence of 2nd frame and was deduced as encoding 312 amino acids.

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Acknowledgement

Supported by : 한국학술진흥재단