Purification and Physicochemical Properties of Superoxide Anion Radical Scavenger from Capsella bursa-pastoris

냉이(Capsella bursa-pastoris)로부터 Superoxide Anion Radical 소거물질의 정제 및 이화학적 성질

  • 곽재혁 (고려대학교 식품공학과) ;
  • 권미향 (고려대학교 생물공학연구소) ;
  • 나경수 (대구공업전문대학 식품영양과) ;
  • 성하진 (고려대학교 유전공학과) ;
  • 양한철 (고려대학교 식품공학과)
  • Published : 1996.02.28

Abstract

A scavenger of superoxide anion radical which causes oxygen toxicity was isolated from Capsella bursa-pastoris, and its physicochemical properties were investigated. The scavenger was isolated and purified by solvent fractionation and liquid column chromatographies (Amberlite XAD-2, Sephadex LH-20, Bio gel P-2, ODS (silica gel with 100% octadecyl silanization)). An active compound of 0.25 g was finally isolated by Fast Protein Liquid chromatography (FPLC) from 100 g ethanol extract of Capsella bursa-pastoris. A 50% decrease of superoxide anion radical was obtained with the scavenger compound of 0.58 g. The compound was assumed to be a phenolic glycoside from its physicochemical properties.