Isolation of 3,4-Dihydroxycinnamic Acid with Antimicrobial Activity from Bark of Aralia elata

두릅수피에서 항미생물 활성을 갖는 3,4-Dihydroxycinnamic Acid의 분리

  • Ma, Seung-Jin (Department of Food Science and Technology, Chonnam National University) ;
  • Kuk, Ju-Hee (Department of Food Science and Technology, Chonnam National University) ;
  • Ko, Byoung-Seob (Korea Institute of Oriental Medicine) ;
  • Park, Keun-Hyung (Department of Food Science and Technology, Chonnam National University)
  • Published : 1996.06.30

Abstract

The methanol extract of Aralia elata bark showed antimicrobial activities against bacteria, yeast and fungi. The active components were successively purified with solvent fractionation, silica gel adsorption column chromatography, Sephadex LH-20 column chromatography, silica gel partition column chromatography and HPLC. The active substances were separated with HPLC where 1% acetic acid-MeOH (60 : 40, v/v) was used as mobile phase. The isolated active substance ($t_r$ 17.1 min) was identified as trans-3,4-dihydroxycinnamic acid by $MS,\;^1H-NMR\;and\;^13C-NMR$.

Keywords

Aralia elata bark;antimicrobial activity;3,4-dihydroxycinnamic acid