Screening of Antithrombotic Peptides from Soybean Paste by the Microplate Method

Microplate법에 의한 된장유래의 항혈전 펩타이드 탐색

  • 손동화 (한국식품개발연구원) ;
  • 이경애 (한국식품개발연구원) ;
  • 김승호 (한국식품개발연구원) ;
  • 안창원 ((주)농심 기술개발연구소) ;
  • 남희섭 ((주)농심 기술개발연구소) ;
  • 이형재 ((주)농심 기술개발연구소) ;
  • 신재익 ((주)농심 기술개발연구소)
  • Published : 1996.08.30

Abstract

In order to search for antithrombotic peptides from soybean paste, the inhibitory activity of water extract of soybean paste and its peptide fractions on ADP-induced aggregation of washed platelets was assayed. Soybean paste extract treated with ultrafiltration (M.W. cut off, 3,000 daltons) was found to have inhibitory activity of 90% at the dose of $96\;{\mu}g/ml$ by the method of turbidometric aggregometer. Soybean paste extract was fractionated to 19 fractions (No. B-18) by Dowex 50W X-2 ion exchange column chromatography and activity test was performed by the microplate method. All of the fractions had antithrombotic activity $(IC_{50},\;101,000\;{\mu}g/ml)$, and most fractions had higher activity than positive control, RGDS $(IC_{50}.\;205\;{\mu}g/ml)$. Especially, basic fractions No.16-18 showed higher activity than soybean paste extract $(IC_{50},\;30\;{\mu}g/ml)$. The fraction No.16 with the highest activity $(IC_{50},\;10\;{\mu}g/ml)$ was purified and analysed for amino acid composition. The results showed that histidine, arginine, and alanine were major residues in the peptide part of the fraction.

Keywords

antithrombotic peptide;soybean paste;microplate method