Antimutagenic Activity in vitro of Chitosan Hydrolysates

Chitosan 가수분해물의 in vitro 항돌연변이 활성

  • Chang, Hyun-Joo (Rice Utilization Research Center, Korea Food Research Institute) ;
  • Chun, Hyang-Sook (Rice Utilization Research Center, Korea Food Research Institute) ;
  • Lee, Su-Rae (Department of Food and Nutrition, Ewha Women's University)
  • 장현주 (한국식품개발연구원 쌀이용연구센터) ;
  • 전향숙 (한국식품개발연구원 쌀이용연구센터) ;
  • 이서래 (이화여자대학교 식품영양학과)
  • Published : 1996.12.30


Antimutagenic effect of chitosan hydrolysates was investigated using Salmonella typhimurium reversion assay and SOS chromotest against 3-amino-1-methyl-5H-pyrido[4,3-b]lindole(Trp-P-2), aflatoxin $B_1$, 2-nitrofluorene and 4-nitroquinoline oxide. After partial acid hydrolysis of chitosan, six fraction of different molecular size were obtained by ultrafiltration. Chitosan hydrolysates showed antimutagenic effect of $0{\sim}78%$ on Trp-P-2, $0{\sim}92%$ on aflatoxin $B_1$ and $0{\sim}51%$ on 2-nitrofluorene in Salmonella typhimurium reversion assay. Inhibitory effect in Salmonella typhimurium reversion assay showed the highest at 5% concentration of fraction 6 in Trp-P-2, 10% concentration of fraction 5 in aflatoxin $B_1$ and 5% concentration of fraction 6 on 2-nitrofluorene. In SOS chromotest, chitosan hydrolysates showed anitimutagenic effect of $0{\sim}54%$ on Trp-P-2 and $0{\sim}77%$ on 4-nitroquinoline oxide, These results suggest that high molecular weight fraction of chitosan hydrolysates (MW>30,000) in most effective to inhibit mutagenicity of tested mutagens.