An Enzyme-Linked Immunosorbent Assay for $Aflatoxin\;M_1$ in Cow's Milk without a Cleanup Procedure

희석에 의한 우유 중 $Aflatoxin\;M_1$의 효소면역측정법

  • Shon, Dong-Hwa (Korea Food Research Institute) ;
  • Lim, Sun-Hee (Research Center for New Bio-Materials in Agriculture, Seoul National University) ;
  • Lee, Yin-Won (Research Center for New Bio-Materials in Agriculture, Seoul National University)
  • 손동화 (한국식품개발연구원) ;
  • 임선희 (농업생물신소재연구센터, 서울대학교 농업생명과학대학) ;
  • 이인원 (농업생물신소재연구센터, 서울대학교 농업생명과학대학)
  • Published : 1996.12.30

Abstract

A simple and rapid detection system for $aflatoxin\;M_1\;(AFM_1)$ in cow's milk by an enzyme-linked immunosorbent assay (ELISA) was developed. Specific antibodies against $AFM_1$, conjugated to bovine serum albumin $(AFM_1-BSA)$ were raised in rabbits and purified. The cross-reactivities of the antibodies against aflatoxin analogs were less than 29.9%. When a competitive direct ELISA (cdELISA) for $AFM_1$, established by use of the antibodies was applied to the spike test of $AFM_1$ onto uncontaminated cow's milk, the assay recovery was unstable unless cow's milk was diluted to 40% (2:3) with phosphate buffered saline (PBS). In that condition of sample dilution, the mean ELISA recovery of $AFM_1$, from the cow's milk was 113% (coefficient of variation (CV) of each recovery percentage, 8.2%) in the range of $0.3{\sim}3.0\;ppb$. These results showed that the ELISA system could be a convenient tool to monitor the contamination of AFM1 more than 0.5 ppb in cow's milk (FDA allowance limit) easily.

Keywords

$aflatoxin\;M_1$;ELISA;cow's milk;without cleanup