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HEMAGGLUTINATION AND COLONY HYBRIDIZATION FOR THE IDENTIFICATION OF ENTEROTOXIGENIC Escherichia coli ISOLATED FROM HEALTHY PIG

  • Choi, S.H. ;
  • Oh, M.J. ;
  • Sung, C.
  • Received : 1996.03.04
  • Accepted : 1996.07.01
  • Published : 1996.12.01

Abstract

Erythrocytes from three different animal species were used to determine mannose-sensitive hemagglutination (MSHA) and mannose-resistant hemagglutination (MRHA) of 755 isolates obtained from rectal swabs of healthy pig. In addition, colony hybridization using digoxigenin-dUTP labeled polynucleotide probes was performed for the detection of heat-stable and heat-labile enterotoxin genes carried by MRHA positive isolates. Of 755 strains, 9, 4 and 28 strains gave a positive MRHA with bovine, equine and pig erythrocytes, respectively. Of these isolates, 28 (3.7%) were characterized for positive MRHA by at least one blood. Seven isolates gave a positive MRHA with two kinds of blood. Three gave a positive MRHA with three kinds of blood. Twenty-eight strains, while positive in MRHA, yielded negative signals in the colony hybridization assay for the detection of heat-stable (STaI and STaII) and heat-labile (LT) enterotoxin genes in E. coli.

Keywords

MSHA;MRHA;Colony Hybridization;Enterotoxigenic E. coli