Improvement of micronucleus assay in the lymphocytes using Argyrophilic nucleolor organizer region(AgNOR) staining

Argyrophilic nucleolar organizer region(AgNOR) 염색을 이용한 림프구의 미세핵분석법의 개선

  • Kim, Sung-ho (College of Veterinary Medicine, Chonnam National University) ;
  • Han, Dong-un (College of Veterinary Medicine, Chonnam National University) ;
  • Kang, Mun-il (College of Veterinary Medicine, Chonnam National University) ;
  • Lim, Jeong-taek (College of Veterinary Medicine, Chonnam National University)
  • Received : 1995.11.24
  • Published : 1996.06.25

Abstract

A technique to improve the analysis of micronuclei(MN) in lymphocytes as a cytogenetic indicator is reported. For the purpose of diminishing the variation of the result from individual reader and making it easier to distinguish accurately a cytokinesis blicked(CB) lymphocyte and micronuclei, we tried a modified one-step silver staining technique as a method for detection of the argyrophilic nucleolar organizer region(AgNOR) with or without conventional Giemsa stain in the slide from CB method. Compared with the conventional Giemsa stain, the preparation processed with this method are especially useful for the accurate analysis of MN of cultured lymphocyte with cytochalasin B. This method will be a useful technique for automated calculation of MN.