Studies on the production and characterization of monoclonal antibodies against bovine rotaviruses isolated in Korea

소 로타바이러스(국내분리주)에 대한 단크론항체 생산 및 특성에 관한 연구

  • Ahn, Jae-moon (Chungbuk Office of Agriculture and Livestock) ;
  • Cho, Sun-hee (College of Veterinary Medicine, Chungbuk National University) ;
  • Kang, Shien-young (College of Veterinary Medicine, Chungbuk National University)
  • Received : 1996.01.15
  • Published : 1996.06.25

Abstract

Monoclonal antibodies(MAbs) against field isolates of the bovine rotavirus A strain(G6), V strain(G10) and reference I-801 strain(G8) were produced and characterized. Six MAbs(4C2, 4D9, 5E1, 5E7, 5D5, 3E4) against A strain had neutralizing activity and reacted only with the G6 bovine rotaviruses determined by fluorescence focus neutralization (FFN) test. Otherwise, five neutralizing MAbs(1G2, 2G6, 5E2, 5E12, 5H7) against I-801 strain neutralized the G6 and G8 bovine rotaviruses. Five non-neutralizing MAbs(5F12, 7F12, 5E11, 2A11, 2B12) were VP6-specific and cross-reacted with all bovine and porcine rotaviruses examined by fluorescence antibody(FA) test. None of the MAbs reacted with bovie viral diarrhea virus(BVDV) and bovine coronavirus(BCV) determined by FA and FFN test.

Acknowledgement

Supported by : 한국학술진흥재단