Effect of Apple Dietary Fiber on the in vitro Growth of Intestinal Bacteria

사과의 식이섬유질이 장내세균의 in vitro 생육에 미치는 영향

  • Lee, Hyeon-Ah (Department of Food and Nutrition, Hanyang University) ;
  • Lee, Sang-Sun (Department of Food and Nutrition, Hanyang University) ;
  • Shin, Hyun-Kyung (Department of Food and Nutrition, Hallym University)
  • 이현아 (한양대학교 식품영양학과) ;
  • 이상선 (한양대학교 식품영양학과) ;
  • 신현경 (한림대학교 식품영양학과)
  • Published : 1997.02.01


Various fractions of apple fibers such as crude pulp, total dietary fiber, soluble dietary fiber, and insoluble dietary fiber were prepared and added to the proteose peptone-yeast extract-fildes (PYF) media to see their effects on the growth of type cultures of intestinal bacteria. Most microbes tested in this experiment grew well in PYF media with the soluble dietary fiber of apple than with the insoluble dietary fiber. Especially Bifidobacterium species such as B. adolescentis, B. animalis, B. infantis, B. longum, B. thermophilum showed higher growth in PYF media containing the soluble dietary fiber than other fiber fractions. However, pectin-added media didn't promote the growth of most microbes used in the experiment. In the in vitro mixed culture using rat feces as starter, the addition of the soluble dietary fiber or pectin to the basal medium showed larger proportion of Bifidobacterium species in total bacteria than that of glucose.