Isolation and Characterization of Benzoic Acid with Antimicrobial Activity from Needle of Pinus densiflora

솔잎에서 항미생물 활성을 갖는 benzoic acid의 분리 및 동정

  • Kuk, Ju-Hee (Department of Food Science and Technology, Chonnam National University) ;
  • Ma, Seung-Jin (Department of Food Science and Technology, Chonnam National University) ;
  • Park, Keun-Hyung (Department of Food Science and Technology, Chonnam National University)
  • Published : 1997.04.01

Abstract

The ethyl acetate (EtOAc) extracts from needles of Pinus densiflora were showed antimicrobial activities against bacteria, yeast and fungi. The antimicrobial active substance of EtOAc extracts were successively purified with solvent fractionation, silica gel adsorption column chromatography and Sephadex LH-20 column chromatography. The purified active substance was isolated as crystals and identified as benzoic acid by $MS,\;^{1}H-NMR\;and\;^{13}C-NMR$. The amount of benzoic acid was 0.608 mg per gram of fresh needle of Pinus densiflora.