Determination of Patulin in Commercial Apple Juice in Korea by High Performance Liquid Chromatography

High Performance Liquid Chromatography를 이용한 국내 시판 사과 주스의 Patulin 분석

  • Cho, Wan-Il (Department of Food Science and Technology, Seoul National University) ;
  • Choi, Young-Boong (Department of Food Science and Technology, Seoul National University) ;
  • Moon, Tae-Wha (Department of Food Science and Technology, Seoul National University)
  • Published : 1997.06.01

Abstract

Seven brands of commercial apple juice in Korea obtained from local stores were analyzed for patulin. Sep-Pak silica cartridge purification of the extract by ethyl acetate offered a good separation of patulin from other components. Patulin was determined by reverse phase liquid chromatography using a J'sphere $4\;{\mu}m$ ODS-H80 column with an ultraviolet detector set at 274 nm. Patulin concentrations in four samples ranged from 4 to $20\;{\mu}g/L$ and the other three samples from 30 to $45\;{\mu}g/L$. The limit of detection was $3\;{\mu}g/L$, and the recovery was $80{\sim}90%$ at the contamination level of $3.78{\sim}125.9\;{\mu}g/L$.

Keywords

patulin;apple juice;high performance liquid chromatography