A Glutamate Oxidase-based Biosensor for the Determination of Glutamate

Glutamate Oxidase를 이용한 Glutamate 측정용 Biosensor의 개발

  • Lee, Young-Chun (Department of Food Science and Technology, Chung-Ang-University) ;
  • Lee, Sang-Hyun (Department of Food and Nutrition, Jang-An Junior College)
  • 이영춘 (중앙대학교 식품공학과) ;
  • 이상현 (장안전문대학 식품영양학과)
  • Published : 1997.12.01


The objective of this research was to develop a glutamate enzyme sensor for rapid determinations of glutamate in samples. Glutamate oxidase was immobilized onto activated nylon, chitosan and other membranes. The enzymic and nonactin membranes were attached to an ammonia electrode to detect ammonia generated by the reaction between glutamate oxidase and glutamate. The enzyme immobilized on activated nylon membrane was stable for 2 months, and was able to perform about 250 glutamate determinations without losing activities. The enzyme immobilized on chitosan membrane had higher enzyme activity, but was not as much stable as that immobilized on nylon. The glutamate biosensor was able to accurately determine $0.1{\sim}5\;mM$ of glutamate in samples.