Studies on development of ELISA Kits for T-2 toxin

T-2 독소의 측정을 위한 ELISA Kits의 개발에 관한 연구

  • Yoon, Hwa-joong (Department of Veterinary Medicine, College of Animal Husbandry, Kon-Kuk University) ;
  • Kim, Taejong (Department of Veterinary Medicine, College of Animal Husbandry, Kon-Kuk University) ;
  • Lee, Sung-yun (Department of Veterinary Medicine, College of Animal Husbandry, Kon-Kuk University)
  • 윤화중 (건국대학교 축산대학 수의학과) ;
  • 김태종 (건국대학교 축산대학 수의학과) ;
  • 이승윤 (건국대학교 축산대학 수의학과)
  • Received : 1997.06.14
  • Published : 1997.09.25

Abstract

Direct competitive enzyme linked immunosorbent assay(ELISA) with monoclonal antibodies have been studied for quantitative determination of T-2 toxin from the mold corn. The T-2HS, T-2HS-BSA, T-2HS-HRP and monoclonal antibodies against T-2 toxin produced in the studies were qualified for quantitative ELISA test of T-2 toxin. The mean recovery rate from ground com spiked T-2 toxin was 83%. The meaning range of the T-2 test was 60ng to $2{\mu}g$. According to the recovery results with the com spiked T-2, the tests proved to be suitable in the screening of the moldy feed samples for the presence of T-2 toxin and will be able to become the basis of the ELISA test for the quantitative screening kits of T-2 toxin.

Acknowledgement

Supported by : 건국대학교