Fabrication of computer-interfaced photon counter for single molecule detection in solution

용액내 단일 분자 검출을 위한 컴퓨터 인터페이싱 광자계수기의 제작

  • Published : 1997.02.01


In order to acquire the fluorescence burst signals emitted from single dye molecules that pass through a detection space defined by a confocal microscope, a computer-interfaced photon counter has been fabricated. The maximum count rate is about 80 MHz, which is limited by the counter devices used. Using both the operating computer program written by BASIC and the 486 PC computer, the minimum bin-width of 25 $mutextrm{s}$ has been achieved. The characteristics of fluorescence burst signals emitted from JA22 molecules at about 1$\times$$10^{-11}$m mol/L in ethylene glycol are discussed briefly.



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