Inhibition of Hepatocellular Carcinoma Cell Growth by the Extract of Symphytum offcinale L. and the Possible Mechanisms for this Inhibition

  • Ham, Seung-Shi (Division of Food and Biotechnology, Kangwon National University) ;
  • Park, Kyong-Gun (Division of Food and Biotechnology, Kangwon National University) ;
  • Lee, Yong-Moon (College of Pharmacy, Chungbuk University) ;
  • Lee, Young-Ik (Genetic Engineering Research Institute) ;
  • Yoon, Ji-Won (Julia McFarlane Diabetes Research Center, Faculty of Medicine, The University of Calgary) ;
  • Kim, Seong-Jin (Laboratory of Chemoprevention, National Cancer Institute) ;
  • Lee, euk-Sik (Division of Food and Biotechnology, Kangwon National University)
  • Published : 1997.09.01

Abstract

A crude extract of Smphytum officinale L. (comfrey) was for its ability to inhibit he growth of hepatocellular carcinoma cells and expression of the insulin-like growth factor I (IGF-II) gene. The DNA synthesis of hepatocellular carcinoma cell lines, Hep G2, Hep 3B, and PLC/PRF/5 was inhibited by a crude extract of Smphytum officinale in both a time- and a dose-dependent manners. This plant extract also inhibited expression of the IGF-II gene. Since IGF-II exerts a mitogenic effect on Hep G2 cells, these results suggest that the growth inhibition by Symphytum officinale extract is, in part, mediated through the inhibition of IGF-II gene expression.