Purification and Characterization of Internal Invertase in Rhodosporidum toruloides Mating Type a Cells

  • Jeong, Youn-Kee (Dept. of Microbiology, Dong eui University) ;
  • Cho, Kyung-Soon (Public Health and Environment Institute of Pusan) ;
  • Lee, Tae-Ho (Dept. of Microbiology, Pusan National University) ;
  • Ryu, Beung-Ho (Dept. of Food Microbiology and Technology, Kyungsung University)
  • Published : 1997.09.01

Abstract

The internal invertase of Rhodosporidium toruloids mating type a cells was purified to a single band on SDS-PAGE from cell-free extract by acid precipitation, ion exchange chromatogaphy andgel filtration. The determined molecular weight of he purified enzyme was about 95,000 by gel filtration and 100,000 daltons on SDS-polyacryamide gel electrophoresis. This enzyme didn't show any activity change by several metal ions except 15.4% decrease by {TEX}$Mn^{2+}${/TEX} and was strongly inhibited by 2-mercaptoethanol and SDS. The invertase maintained its activity at high level until 70℃, but inactivated at 80℃ almost completely. The optimal temperature and pH of the enzyme were about 60℃ and pH 5.0, respectively. The stable pH range of invertase was narrow from pH 3.0 to 6.0. The Km value and isoelectric point of enzyme were 3.4×{TEX}$10^{3}${/TEX} M, pH 4.4, respectively.