Simultaneous Determination of Berberine, Cinnamic Acid and Glycyrrhizin in Pharmaceutical Formulations by Capillary Electrophoresis with Diode-Array Detection

모세관 전기이동법에 의한 생약제제중 베르베린, 계피산 및 글리시리진의 동시 정량

  • Kang, Seong Ho (Department of Chemistry, Seoul National University) ;
  • Chung, Wha Jin (Central Research Institute, Hanil Pharmaceutical Ind, Co., LTD) ;
  • Yoon, Hyung Jung (Central Research Institute, Hanil Pharmaceutical Ind, Co., LTD) ;
  • Chung, Doo Soo (Department of Chemistry, Seoul National University)
  • Published : 19970200

Abstract

A simple, accurate and reproducible capillary electrophoresis(CE) assay has been developed for the determination of berberine, cinnamic acid, and glycyrrhizin which are used in traditional Korean medicinal preparations. Separation of these compounds was performed in 20 mM phosphate buffer(pH 7.5) and acetonitrile(75:25, v/v) using a bare fused silica capillary($57 cm{\times}75 {\mu}m$ i.d.) at 25$^{\circ}C$. With the electric field of 350 V/cm, the time needed for the separation of berberine, cinnamic acid and glycyrrhizin was within 13 min. Calibration curves were linear for 1∼100 ${\mu}g/mL$ berberine, 0.3∼100 ${\mu}g/mL$ cinnamic acid and 2.5∼100 ${\mu}g/mL$ glycyrrhizin. The ranges of relative standard deviations(n=5) for those samples were between 0.96∼2.35%. The limits of detection(S/N=3) for berberine, cinnamic acid and glycyrrhizin were 0.5, 0.1 and 2.0 ${\mu}g/mL$, respectively. The numbers of theoretical plates were 181,000(berberine), 88,000(cinnamic acid) and 169,000(glycyrrhizin), while they were 3,100∼4,800 in HPLC.

Keywords

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