A Study on the Analysis of Histamine by Spectrofluorimetry

형광측정법을 이용한 히스타민 분석방법 개발 연구

  • 이재성 (한국과학기술연구원 특성분석센터) ;
  • 김영상 (고려대학교 화학과) ;
  • 차기석 (한국과학기술연구원 특성분석센터) ;
  • 이희정 (고려대학교 화학과) ;
  • 홍천수 (연세대학교 의과대학 내과학교실) ;
  • 남혜원 (수원여자전문대학 식품영양학과) ;
  • 명춘옥 (오산전문대학 식품조리학과) ;
  • 이기완 (경원대학교 식품영양학과) ;
  • 이양자 (연세대학교 식품영양학과)
  • Received : 1996.10.04
  • Published : 1997.02.25

Abstract

The application of high performance liquid chromatogaphy(HPLC) with fluorimetric detection for the measurement of histamine in biological fluids has been developed recently. Because of time consumption during measurement and difficulties in controling optimum operating condition for HPLC system, spectrofluorimetry was adopted to detect histamine. Detection limit by spectrofluorimetry was $3.0{\times}10^{-2}{\mu}g/mL$ wi th R. S. D. 3.74%~16.2% on standard solution. The relative ratio of histmine levels between the result from HPLC-fluorimetry and that from spectrofluorimetry ranged from 1.5 to 3.5. The recoveries of each samples obtained from standard addition method by spctrofluorimetry have shown 100%~125%.

Keywords

Histamine;Spectrofluorimetry