Effect of Oxygen Composition on Polyhydroxybutyrate Synthesis by Alcaligenes eutrophus at Various Pressures

Alcaligenes eutrophus에 의한 Polyhydroxybutyrate의 합성에 관한 산소효과

  • 김교근 (청주대학교 이공대학 환경공학과) ;
  • 신선경 (국립환경연구원 환경보건부 미량물질분석과) ;
  • 권효식 (충북대학교 사범대학 과학교육과)
  • Received : 1996.08.01
  • Published : 1997.02.25

Abstract

Poly-${\beta}$-hydroxybutyrates(PHB) are well known intracellular biopolymer which are completely degraded to carbon dioxide and water in the landfill. The pressurized fermentation method was used to increase the cell growth of Alcaligenes eutrophus and productivity of PHB. The experimental data were analyzed in terms of carbon source and gas composition effects at various elevated pressures and temperatures. The results reveal that the flow rate of hydrogen gas of 0.0075vvm for the culture is better PHB production than the no hydrogen flow when fructose was used as a sole carbon source. The higher yields and productivities of PHB biosynthesized by A. eutrophus were obtained when the oxygen composition was changed from 2% to 8% at 6atm and $30^{\circ}C$.