Action of P2X-purinoceptor on urinary bladder smooth muscle of pig

돼지 방광 평활근에 있어서 P2X-purinoceptor의 작용

  • Park, Sang-eun (Pusan branch, National Animal Quarantine Service) ;
  • Hong, Yong-geun (College of Veterinary Medicine and Institute of Animal Medicine Gyeong National University) ;
  • Shim, Cheol-soo (Eastern branch, Gyeongnam Livestock Promotion Institufe) ;
  • Jeon, Seok-cheol (Cardiac Center, Masan Samsung General Hospital) ;
  • Kim, Joo-heon (College of Veterinary Medicine and Institute of Animal Medicine Gyeong National University)
  • 박상은 (국립동물검역소 부산지소) ;
  • 홍용근 (경상대학교 수의과대학 동물의학연구소) ;
  • 심철수 (경상남도 축산진흥연구소 동부지소) ;
  • 전석철 (마산삼성병원 심장센터) ;
  • 김주헌 (경상대학교 수의과대학 동물의학연구소)
  • Received : 1995.07.15
  • Published : 1997.03.25

Abstract

The experiments were carried out to elucidate the relationships between neurogenic effects of electrical transmural nerve stimulation and effect of adenosine 5'-triphosphate(ATP) to purinoceptor on the urinary bladder smooth muscle of pig. The results were as follows : 1. The contractile responses induced by electrical transmural nerve stimulation(10V or 20V, 0.5msec, 10sec) were the frequency(2~64Hz) dependent manner. 2. The contractile response induced by carbachol was responsed with a dose-dependent manner and the maximum contractility was $10^{-4}M$. 3. The contractile responses induced by ATP were increased in a dose-dependent manner ($10^{-5}{\sim}10^{-3}M$). 4. The contractile response induced by electrical transmural nerve stimulation(10V, 2~32Hz, 0.5msec, 10sec) was partially blocked by the treatment with atropine($10^{-5}M$), and was powerfully inhibited by 3 times of addition with ATP($10^{-5}M$). 5. The contractile response induced by electrical transmural nerve stimulation(10V, 2~32Hz, 0.5msec, 10sec) was partially blocked by the treatment with atropine($10^{-5}M$), and was completely blocked by the desensitization of the $P_{2X}$-purinoceptor using ${\alpha}$, ${\beta}$-methylene ATP($5{\times}10^{-5}M$). These results suggest that purinergic nerve was innervated, and ATP and acetylcholine was released by the electrical transmural nerve stimulation in urinary bladder smooth muscle of pig.