Comparison of enzyme cytochemical activities between rosetted cells in peripheral blood of Korean native cattle

한우(韓牛) 말초혈액내(末梢血液內) rosette 형성세포(形成細胞)의 효소화학적(酵素化學的) 염색성(染色性) 비교(比較)

  • Kim, Nyeun-su (Chung Bu Branch of the Kang Won-Do Veterinary Service Laboratory) ;
  • Song, Hee-jong (College of Veterinary Medicine, Chonbuk National University) ;
  • Kim, Sun-jae (Department of Veterinary Medicine, Kon-Kuk University) ;
  • Seo, Ye-won (Chung Bu Branch of the Kang Won-Do Veterinary Service Laboratory)
  • 김연수 (강원도 가축위생시험소 중부지소) ;
  • 송희종 (전북대학교 수의과대학) ;
  • 김순재 (건국대학교 수의학과) ;
  • 서예원 (강원도 가축위생시험소 중부지소)
  • Received : 1996.04.12
  • Published : 1997.03.25

Abstract

Peripheral blood mononuclear cells(PBMNC) of Korean native cattle rosetted with Korean goat erythrocytes(KGRBC) and blood monocytes were evaluated for four cytochemical reactions such as acid phosphatase(ACP), alkaline phosphatase-anti-boby(ALP-Ab), ${\alpha}$-naphthyl butyrate esterase(${\alpha}$-NBE) and peroxidase. The results obtained were as follows; In rosetted cells, the positivities of ACP in E AET-DeX, EA and EAC were 70.3%, 22.4% and 25.2%, those of ${\alpha}$-NB were 27.4%, 44.2% and 79.8%, and those of ALP-Ab were 9.5%, 88.3% and 91.5%, respectively. Whereas, the positivity for Peroxidase in monocytes was 100%. In non-rosetted (remained) cells, the positivities of ACP in E AET+DeX. EA and EAC were 41.4%, 57.2% and 61.9%, those of ${\alpha}$-NB were 38.6%, 16.5% and 18.9% and those of ALP-Ab were 98.2%, 5.3% and 6.3%, in order.